The following is the entry list for the first race of the NASCAR Cup Series of the 2025 season – The Daytona 500.
| Car Number | Driver | CIELO Cup Series Rating |
| 01 | Corey LaJoie | 1409 |
| 1 | Ross Chastain | 1696 |
| 2 | Austin Cindric | 1749 |
| 3 | Austin Dillon | 1603 |
| 4 | Noah Gragson | 1760 |
| 5 | Kyle Larson | 1985 |
| 6 | Brad Keselowski | 1736 |
| 7 | Justin Haley | |
| 8 | Kyle Busch | 1592 |
| 9 | Chase Elliott | 1894 |
| 10 | Ty Dillon | 1581 |
| 11 | Denny Hamlin | 1938 |
| 12 | Ryan Blaney | 1941 |
| 16 | AJ Allmendinger | 1778 |
| 17 | Chris Buescher | 1750 |
| 19 | Chase Briscoe | 1594 |
| 20 | Christopher Bell | 1934 |
| 21 | Josh Berry | 1629 |
| 22 | Joey Logano | 1878 |
| 23 | Bubba Wallace | 1831 |
| 24 | William Byron | 2028 |
| 34 | Todd Gilliland | 1596 |
| 35 | Riley Herbst | 1352 |
| 38 | Zane Smith | 1472 |
| 40 | Justin Allgaier | 1603 |
| 41 | Cole Custer | 1535 |
| 42 | John Hunter Nemechek | 1473 |
| 43 | Erik Jones | 1604 |
| 44 | JJ Yeley | 1247 |
| 45 | Tyler Reddick | 1731 |
| 47 | Ricky Stenhouse Jr | 1533 |
| 48 | Alex Bowman | 1806 |
| 51 | Cody Ware | 1737 |
| 54 | Ty Gibbs | 1330 |
| 56 | Martin Truex Jr. | 1688 |
| 60 | Ryan Preece | 1556 |
| 62 | Anthony Alfredo | 1613 |
| 66 | Chandler Smith | 1744 |
| 71 | Michael McDowell | 1503 |
| 77 | Carson Hocevar | 1689 |
| 78 | BJ McLeod | 1510 |
| 84 | Jimmie Johnson | 1426 |
| 88 | Shane van Gisbergen | 1735 |
| 91 | Hélio Castroneves | |
| 99 | Daniel Suárez | 1750 |
(sps – 2025-02-12)